Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation
نویسندگان
چکیده
منابع مشابه
Saturated patterned excitation microscopy with two-dimensional excitation patterns.
The techniques of patterned excitation microscopy (PEM, also referred to in the literature as structured illumination, harmonic excitation light microscopy, or laterally modulated excitation microscopy), has recently been extended to the non-linear regime, permitting a further increase in resolution breaking the Abbe diffraction limit (saturated PEM, saturated patterned excitation microscopy (S...
متن کاملThree-dimensional super-resolution imaging by stochastic optical reconstruction microscopy.
Recent advances in far-field fluorescence microscopy have led to substantial improvements in image resolution, achieving a near-molecular resolution of 20 to 30 nanometers in the two lateral dimensions. Three-dimensional (3D) nanoscale-resolution imaging, however, remains a challenge. We demonstrated 3D stochastic optical reconstruction microscopy (STORM) by using optical astigmatism to determi...
متن کاملA guide to super-resolution fluorescence microscopy
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new ap...
متن کاملHigh-resolution fluorescence microscopy using three-dimensional structured illumination
We developed a high-resolution microscope based on three-dimensional structured illumination generated with two spatial light modulators. This setup enables both lateral resolution improvement by a factor two and axial localization of point like objects with nanometric precision.
متن کاملNanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy.
We report a noninvasive fluorescence microscopy method and demonstrate nanometer resolution along the optical axis. The technique is based on the influence of the microscope slide on the angular intensity distribution of fluorescence. Axial positions are determined by measuring the proportion of light emitted below the critical angle of total internal reflection, which behaves in a classical wa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-019-09297-5